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WB analysis of human small intestine tissue using GTX46619 SI antibody at 0.2-1microg/ml.
WB analysis of human small intestine tissue using GTX46619 SI antibody at 0.2-1microg/ml.
WB analysis of human small intestine tissue using GTX46619 SI antibody at 0.2-1microg/ml.

SI antibody, Internal

GTX46619
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSI
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Overview

  • Supplier
    GeneTex
  • Product Name
    SI antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6476
  • Target name
    SI
  • Target description
    sucrase-isomaltase
  • Target synonyms
    alpha-glucosidase; Alpha-methylglucosidase; Oligo-1,6-glucosidase; oligosaccharide alpha-1,6-glucosidase; sucrase-isomaltase, intestinal
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14410
  • Protein Name
    Sucrase-isomaltase, intestinal
  • Scientific Description
    This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.[provided by RefSeq, Apr 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SI using anti-SI antibody (A04542-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human COLO-320 whole cell lysate, Lane 3: human SHG-44 whole cell lysate, Lane 4: human HEK293 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SI antigen affinity purified polyclonal antibody (Catalog # A04542-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SI at approximately 209KD. The expected band size for SI is at 209KD.
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