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ProductsBack/SI / Sucrase Isomaltase Antibody (aa1717-1827, Biotin)

SI / Sucrase Isomaltase Antibody (aa1717-1827, Biotin)

LS-C300877
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSI
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SI / Sucrase Isomaltase Antibody (aa1717-1827, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID6476
  • Target name
    SI
  • Target description
    sucrase-isomaltase
  • Target synonyms
    alpha-glucosidase; Alpha-methylglucosidase; Oligo-1,6-glucosidase; oligosaccharide alpha-1,6-glucosidase; sucrase-isomaltase, intestinal
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SI using anti-SI antibody (A04542-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human COLO-320 whole cell lysate, Lane 3: human SHG-44 whole cell lysate, Lane 4: human HEK293 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SI antigen affinity purified polyclonal antibody (Catalog # A04542-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SI at approximately 209KD. The expected band size for SI is at 209KD.
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