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ProductsBack/Sigma Receptor (B-5): m-IgG Fc BP-HRP Bundle

Sigma Receptor (B-5): m-IgG Fc BP-HRP Bundle

SC-528853
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSIGMAR1
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Sigma Receptor (B-5): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10280
  • Target name
    SIGMAR1
  • Target description
    sigma non-opioid intracellular receptor 1
  • Target synonyms
    ALS16, DSMA2, HMNR2, OPRS1, SIG-1R, SR-BP, SR-BP1, SRBP, hSigmaR1, sigma1R, sigma non-opioid intracellular receptor 1, SR31747 binding protein 1, aging-associated gene 8 protein, sigma 1-type opioid receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human COLO-320 whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: monkey COS-7 whole cell lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sigma1-Receptor/SIGMAR1 antigen affinity purified polyclonal antibody (Catalog # A02493-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sigma1-Receptor/SIGMAR1 at approximately 25 kDa. The expected band size for Sigma1-Receptor/SIGMAR1 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband(r)A02493-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetSIGMAR1
  • SizePrice
Product group Antibodies
Abbexa
OPRS1 AntibodyABX031685
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SIGMAR1 Antibody144-05479
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSIGMAR1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OPRS1 Polyclonal Antibody, Unconjugated (bs-5111R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
OPRS1 Polyclonal AntibodyBS-5111R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetSIGMAR1
  • SizePrice
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA021320LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
SIGMAR1 AntibodyCSB-PA021320LA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSIGMAR1
  • SizePrice
Product group Antibodies
Assay Genie
SIGMAR1 AntibodyCAB5479
ReactivityHuman
TargetSIGMAR1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
OPRS1 / SIGMAR1 AntibodyLS-C401334
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSIGMAR1
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Immunohistochemistry analysis in human liver and skeletal muscle tissues using HPA024071 antibody. Corresponding SIGMAR1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-SIGMAR1 AntibodyHPA024071
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSIGMAR1
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Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [N1C3] (GTX115389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
SIGMAR1 antibody [N1C3]GTX115389
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetSIGMAR1
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Santa Cruz Biotechnology
Sigma Receptor (F-5)SC-166392
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ReactivityHuman, Mouse, Rat
TargetSIGMAR1
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