Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2], N-term (GTX116326). Western blot analysis was performed using SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
![SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116326/GTX116326_40282_20160127_IFA_w_23060519_321.webp "SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.")
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116326/GTX116326_40282_20241206_WB_24121100_968.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2], N-term (GTX116326). Western blot analysis was performed using SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
SIN3B antibody [N1N2], N-term
GTX116326
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSIN3B
Overview
- SupplierGeneTex
- Product NameSIN3B antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.96 mg/ml
- ConjugateUnconjugated
- Gene ID23309
- Target nameSIN3B
- Target descriptionSIN3 transcription regulator family member B
- Target synonymspaired amphipathic helix protein Sin3b, SIN3 homolog B, transcriptional regulator, SIN3 transcription regulator homolog B, histone deacetylase complex subunit Sin3b, transcriptional corepressor Sin3b
- HostRabbit
- IsotypeIgG
- Protein IDO75182
- Protein NamePaired amphipathic helix protein Sin3b
- Scientific DescriptionActs as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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