SIRT1 Antibody

Biorbyt

SIRT1 Antibody

10

Optimal dilution of the SIRT1 antibody should be determined by the researcher.

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA

ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml ELISA, FACS, IF, WB

Research Use Only

Monoclonal

PCRP-SIRT1-1E11

Unconjugated

SIRT1

sirtuin 1

SIR2, SIR2L1, SIR2alpha, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, SIR2-like protein 1, regulatory protein SIR2 homolog 1, sirtuin type 1

Mouse

IgG1

NAD-dependent protein deacetylase sirtuin-1

The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent matingtype loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Human

-20°C,2°C to 8°C

12352203