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Goat anti-SIRT3 Antibody

ORB19075
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSIRT3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-SIRT3 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35kDa and 48kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:128000, WB: 0.1-0.3 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23410
  • Target name
    SIRT3
  • Target description
    sirtuin 3
  • Target synonyms
    SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, NAD-dependent deacetylase sirtuin-3, mitochondrial, NAD-dependent protein delactylase sirtuin-3, SIR2-like protein 3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, regulatory protein SIR2 homolog 3, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin type 3
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to SIRT3
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT3 using anti-SIRT3 antibody (A01061-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 43 kDa. The expected band size for SIRT3 is at 43 kDa.
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Lane 1: Mouse Liver lysates; Lane 2: Mouse Kindey lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: MouseHeart lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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