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Western Blot analysis of COLO205 cells using Six3/6 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Six3/6 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Six3/6 Polyclonal Antibody

SIX6 Antibody

CSB-PA004096
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSIX6
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Overview

  • Supplier
    Cusabio
  • Product Name
    SIX6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4990
  • Target name
    SIX6
  • Target description
    SIX homeobox 6
  • Target synonyms
    MCOPCT2, ODRMD, OPTX2, Six9, homeobox protein SIX6, homeodomain protein OPTX2, optic homeobox 2, sine oculis homeobox homolog 6, sine oculis homeobox protein 6, sine oculis homeobox-like protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95475
  • Protein Name
    Homeobox protein SIX6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SIX6 using anti-SIX6 antibody (A06502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX6 antigen affinity purified polyclonal antibody (Catalog # A06502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX6 at approximately 28KD. The expected band size for SIX6 is at 28KD.
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