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ICC/IF analysis of HepG2 cells using GTX87996 SIX6 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87996 SIX6 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87996 SIX6 antibody. The picture on the right is blocked with the synthesized peptide.

SIX6 antibody

GTX87996
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetSIX6
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Overview

  • Supplier
    GeneTex
  • Product Name
    SIX6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. ICC/IF: 1:100~1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4990
  • Target name
    SIX6
  • Target description
    SIX homeobox 6
  • Target synonyms
    homeobox protein SIX6; homeodomain protein OPTX2; MCOPCT2; ODRMD; optic homeobox 2; OPTX2; sine oculis homeobox homolog 6; sine oculis homeobox protein 6; sine oculis homeobox-like protein 6; Six9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95475
  • Protein Name
    Homeobox protein SIX6
  • Scientific Description
    The protein encoded by this gene is a homeobox protein that is similar to the Drosophila sine oculis gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2). [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIX6 using anti-SIX6 antibody (A06502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX6 antigen affinity purified polyclonal antibody (Catalog # A06502-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX6 at approximately 28KD. The expected band size for SIX6 is at 28KD.
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