More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SKA2 Antibody
IHC image of CSB-PA823890LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA823890LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA823890LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

SKA2 Antibody

CSB-PA823890LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSKA2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    SKA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID348235
  • Target name
    SKA2
  • Target description
    spindle and kinetochore associated complex subunit 2
  • Target synonyms
    FAM33A, spindle and kinetochore-associated protein 2, family with sequence similarity 33, member A, spindle and KT (kinetochore) associated 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WVK7
  • Protein Name
    Spindle and kinetochore-associated protein 2
  • Scientific Description
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bioss
SKA2 Polyclonal AntibodyBS-7847R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • SizePrice
Anti-SKA2 Antibody
Product group Antibodies
Antibodies.com
Anti-SKA2 AntibodyA49681
ApplicationsWestern Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of SKA2 using anti-SKA2 antibody (A06326). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKA2 antigen affinity purified polyclonal antibody (Catalog # A06326) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKA2 at approximately 20KD. The expected band size for SKA2 is at 14KD.
Product group Antibodies
Boster Bio
Anti-SKA2 Antibody Picoband(r)A06326-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
TargetSKA2
  • SizePrice
WB analysis of 3T3 cell lysate using GTX85247 SKA2 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
Product group Antibodies
GeneTex
SKA2 antibodyGTX85247
ApplicationsWestern Blot, ELISA
TargetSKA2
  • SizePrice
Product group Antibodies
Assay Genie
SKA2 AntibodyPACO59253
ApplicationsELISA, ImmunoHistoChemistry
TargetSKA2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SKA2 Antibody (FITC)LS-C681339
ApplicationsELISA
TargetSKA2
  • SizePrice
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-SKA2 AntibodyHPA059235
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSKA2
  • SizePrice