HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SKI antibody (GTX133764) diluted at 1:1000 and competitor's antibody (sc-9140) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SKI antibody (GTX133764) diluted at 1:1000 and competitor's antibody (sc-9140) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SKI antibody
GTX133764
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSKI
Overview
- SupplierGeneTex
- Product NameSKI antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.29 mg/ml
- ConjugateUnconjugated
- Gene ID6497
- Target nameSKI
- Target descriptionSKI proto-oncogene
- Target synonymsSGS, SKV, ski oncogene, Sloan-Kettering Institute proto-oncogene, proto-oncogene c-Ski, ski oncoprotein, v-ski avian sarcoma viral oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP12755
- Protein NameSki oncogene
- Scientific DescriptionThis gene encodes the nuclear protooncogene protein homolog of avian sarcoma viral (v-ski) oncogene. It functions as a repressor of TGF-beta signaling, and may play a role in neural tube development and muscle differentiation. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Gori I, George R, Purkiss AG, et al. Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-β responses through SKI stabilization. Elife. 2021,10. doi: 10.7554/eLife.63545Read this paper
Datasheet
Related products
Product group Antibodies
Anti-SKI Antibody144-65600
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSKI
- SizePrice
Product group Antibodies
SKI Polyclonal AntibodyBS-1684R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Rat
TargetSKI
- SizePrice
Product group Antibodies
SKI AntibodyCSB-PA960678
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSKI
- SizePrice
Product group Antibodies
ApplicationsWestern Blot
ReactivityEquine, Human
TargetSKI
- SizePrice
Product group Antibodies
SKI antibodyGTX85522
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetSKI
- SizePrice
Product group Antibodies
SKI AntibodyLS-C770628
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSKI
- SizePrice
Product group Antibodies
Anti-SKI AntibodyA91334
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
- SizePrice