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Western Blot analysis of Jurkat NIH-3T3 K562 cells using Skp2 p45 Polyclonal Antibody
Western Blot analysis of Jurkat NIH-3T3 K562 cells using Skp2 p45 Polyclonal Antibody
Western Blot analysis of Jurkat NIH-3T3 K562 cells using Skp2 p45 Polyclonal Antibody

SKP2 Antibody

CSB-PA004098
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSKP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SKP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6502
  • Target name
    SKP2
  • Target description
    S-phase kinase associated protein 2
  • Target synonyms
    CDK2/cyclin A-associated protein p45; FBL1; F-box/LRR-repeat protein 1; FBXL1; FLB1; p45; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13309
  • Protein Name
    S-phase kinase-associated protein 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated(bs-1096R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
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Figure 1. Western blot analysis of SKP2 using anti-SKP2 antibody (A00544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (Catalog # A00544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 48KD. The expected band size for SKP2 is at 48KD.
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