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SKP2 Antibody (YA1332, Rabbit)

HY-P81587
MedChem Express
TargetSKP2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SKP2 Antibody (YA1332, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6502
  • Target name
    SKP2
  • Target description
    S-phase kinase associated protein 2
  • Target synonyms
    FBL1, FBXL1, FLB1, p45, S-phase kinase-associated protein 2, CDK2/cyclin A-associated protein p45, F-box/LRR-repeat protein 1, S-phase kinase-associated protein 2, E3 ubiquitin protein ligase, p45skp2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SKP2 Antibody (YA1332) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1332), targeting SKP2, with a predicted molecular weight of 48 kDa (observed band size: 48 kDa). SKP2 Antibody (YA1332) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SKP2 using anti-SKP2 antibody (A00544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (Catalog # A00544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 48KD. The expected band size for SKP2 is at 48KD.
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