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ProductsBack/SLC12A1 / NKCC2 Antibody (aa351-400)

SLC12A1 / NKCC2 Antibody (aa351-400)

LS-C40323
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC12A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC12A1 / NKCC2 Antibody (aa351-400)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (0.2 - 1 µg/ml) IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6557
  • Target name
    SLC12A1
  • Target description
    solute carrier family 12 member 1
  • Target synonyms
    BSC, BSC-1, BSC1, CCC2, NKCC2, solute carrier family 12 member 1, Na-K-2Cl cotransporter, Na-K-2Cl cotransporter 2, bumetanide-sensitive cotransporter type 1, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, kidney-specific Na-K-Cl symporter, solute carrier family 12 (sodium/potassium/chloride transporter), member 1, solute carrier family 12 (sodium/potassium/chloride transporters), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC12A1 using anti-SLC12A1 antibody (A01106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey kidney tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A1 at approximately 150-290 kDa. The expected band size for SLC12A1 is at 121 kDa.
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