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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA258920(SLC14A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA258920(SLC14A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA258920(SLC14A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

SLC14A1 Antibody

CSB-PA258920
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSLC14A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC14A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6563
  • Target name
    SLC14A1
  • Target description
    solute carrier family 14 member 1 (Kidd blood group)
  • Target synonyms
    HsT1341; HUT11; JK; Jk(b); Kidd blood group antigen; RACH1; RACH2; SLC14A1 JK; solute carrier family 14 (urea transporter), member 1 (Kidd blood group); urea transporter 1; urea transporter JK glycoprotein; urea transporter, erythrocyte; urea transporter-B1; UT1; UT-B1; UTE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13336
  • Protein Name
    Urea transporter 1
  • Scientific Description
    The protein encoded by this gene is a membrane transporter that mediates urea transport in erythrocytes. This gene forms the basis for the Kidd blood group system. Mediates urea transport in erythrocytes By similarity. Low-affinity facilitative urea transporter that allow rapid equilibration between the urinary space and the hyperosmotic interstitium. the rate of urea conduction is increased by hypoosmotic stress.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UT-B/SLC14A1 using anti-UT-B/SLC14A1 antibody (A04926-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UT-B/SLC14A1 antigen affinity purified polyclonal antibody (Catalog # A04926-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UT-B/SLC14A1 at approximately 65 kDa. The expected band size for UT-B/SLC14A1 is at 43 kDa.
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