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SLC17A5 Antibody

LS-C750394
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSLC17A5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC17A5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/54kDa, while the observed MW by Western blot was 31kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 30kDa/54kDa, while the observed MW by Western blot was 31kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26503
  • Target name
    SLC17A5
  • Target description
    solute carrier family 17 member 5
  • Target synonyms
    AST, ISSD, NSD, SD, SIALIN, SIASD, SLD, VEAT, sialin, H(+)/nitrate cotransporter, H(+)/sialic acid cotransporter, acidic sugar transporter, infantile sialic acid storage disorder, membrane glycoprotein HP59, sialic acid storage disease, sodium/sialic acid cotransporter, solute carrier family 17 (acidic sugar transporter), member 5, solute carrier family 17 (anion/sugar transporter), member 5, vesicular H(+)/Aspartate-glutamate cotransporter, vesicular excitatory amino acid transporter
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC17A5 using anti-SLC17A5 antibody (A06657-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse HBZY-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC17A5 antigen affinity purified polyclonal antibody (Catalog # A06657-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC17A5 at approximately 36 kDa. The expected band size for SLC17A5 is at 55 kDa.
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