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ProductsBack/SLC19A1 (D-4): m-IgGkappa BP-HRP Bundle

SLC19A1 (D-4): m-IgGkappa BP-HRP Bundle

SC-523748
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSLC19A1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    SLC19A1 (D-4): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6573
  • Target name
    SLC19A1
  • Target description
    solute carrier family 19 member 1
  • Target synonyms
    CHMD, FOLT, IFC-1, IFC1, IMD114, MEGAF, REFC, RFC, RFC1, RFT-1, hRFC, hSLC19A1, reduced folate transporter, cyclic dinucleotide:anion antiporter SLC19A1, folate transporter 1, folate:anion antiporter SLC19A1, intestinal folate carrier 1, placental folate transporter, reduced folate carrier 1, reduced folate carrier protein, reduced folate transporter 1, solute carrier family 19 (folate transporter), member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RFC/SLC19A1 using anti-RFC/SLC19A1 antibody (A01679-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC/SLC19A1 antigen affinity purified polyclonal antibody (Catalog # A01679-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC/SLC19A1 at approximately 65 kDa. The expected band size for RFC/SLC19A1 is at 65 kDa.
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