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IHC-P analysis of human colon adenocarcinoma tissue using GTX04583 SLC20A1 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL
IHC-P analysis of human colon adenocarcinoma tissue using GTX04583 SLC20A1 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL
IHC-P analysis of human colon adenocarcinoma tissue using GTX04583 SLC20A1 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL

SLC20A1 antibody

GTX04583
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC20A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC20A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.25-0.5 microg/mL. IHC-P: 2-5 microg/mL. ELISA: 0.1-0.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6574
  • Target name
    SLC20A1
  • Target description
    solute carrier family 20 member 1
  • Target synonyms
    GLVR1, Glvr-1, PIT1, PiT-1, sodium-dependent phosphate transporter 1, gibbon ape leukemia virus receptor 1, leukemia virus receptor 1 homolog, solute carrier family 20 (phosphate transporter), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WUM9
  • Protein Name
    Sodium-dependent phosphate transporter 1
  • Scientific Description
    The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism, signal transduction, and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.[provided by RefSeq, Mar 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC20A1 antigen affinity purified polyclonal antibody (Catalog # A03537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC20A1 at approximately 85 kDa. The expected band size for SLC20A1 is at 74 kDa.
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