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Western Blot analysis of HT29 cells using OCT3 Polyclonal Antibody
Western Blot analysis of HT29 cells using OCT3 Polyclonal Antibody
Western Blot analysis of HT29 cells using OCT3 Polyclonal Antibody

SLC22A3 Antibody

CSB-PA003508
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC22A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC22A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6581
  • Target name
    SLC22A3
  • Target description
    solute carrier family 22 member 3
  • Target synonyms
    EMT, EMTH, OCT3, solute carrier family 22 member 3, EMT organic cation transporter 3, extraneuronal monoamine transporter, organic cation transporter 3, solute carrier family 22 (extraneuronal monoamine transporter), member 3, solute carrier family 22 (organic cation transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75751
  • Protein Name
    Solute carrier family 22 member 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (A04914-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # A04914-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD.
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