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SLC22A3 Antibody (YA2830, Rabbit)

HY-P83085
MedChem Express
TargetSLC22A3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SLC22A3 Antibody (YA2830, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6581
  • Target name
    SLC22A3
  • Target description
    solute carrier family 22 member 3
  • Target synonyms
    EMT, EMTH, OCT3, solute carrier family 22 member 3, EMT organic cation transporter 3, extraneuronal monoamine transporter, organic cation transporter 3, solute carrier family 22 (extraneuronal monoamine transporter), member 3, solute carrier family 22 (organic cation transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SLC22A3 Antibody (YA2830) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2830), targeting SLC22A3, with a predicted molecular weight of 61 kDa (observed band size: 75 kDa). SLC22A3 Antibody (YA2830) can be used for WB, IHC-P, FC experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (A04914-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # A04914-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD.
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