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SLC22A8 / OAT3 Antibody (N-Terminus)

LS-C17011
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSLC22A8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC22A8 / OAT3 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10000-1:50000. Coat ELISA plates at 1 ug/ml of Control Peptide.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000) Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10000-1:50000. Coat ELISA plates at 1 ug/ml of Control Peptide.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9376
  • Target name
    SLC22A8
  • Target description
    solute carrier family 22 member 8
  • Target synonyms
    hOAT3; OAT3; organic anion transporter 3; solute carrier family 22 (organic anion transporter), member 8; solute carrier family 22 member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/SLC22A8 antigen affinity purified polyclonal antibody (Catalog # A04087-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/SLC22A8 at approximately 62 kDa. The expected band size for OAT3/SLC22A8 is at 60 kDa.
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