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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA811607LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA811607LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA811607LA01HU at dilution of 1:100

SLC22A9 Antibody

CSB-PA811607LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC22A9
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC22A9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114571
  • Target name
    SLC22A9
  • Target description
    solute carrier family 22 member 9
  • Target synonyms
    HOAT4, OAT4, OAT7, UST3H, ust3, organic anion transporter 7, organic anion transporter 4, organic anion/short-chain fatty acid exchanger, solute carrier family 22 (organic anion transporter), member 9, solute carrier family 22 (organic anion/cation transporter), member 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IVM8
  • Protein Name
    Organic anion transporter 7
  • Scientific Description
    Sodium-independent organic anion transporter which exhibits high specificity for sulfated conjugates of xenobiotics and steroid hormones. It is also specifically activated by 3 to 5 carbons-containing short-chain fatty acids/SCFAs, including propionate, butyrate and valerate. May operate the exchange of sulfated organic components against short-chain fatty acids/SCFAs at the sinusoidal membrane of hepatocytes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC22A9 using anti-SLC22A9 antibody (A08762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A9 antigen affinity purified polyclonal antibody (Catalog # A08762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A9 at approximately 70 kDa. The expected band size for SLC22A9 is at 62 kDa.
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