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ProductsBack/SLC22A9 / OAT7 Antibody

SLC22A9 / OAT7 Antibody

LS-C497141
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC22A9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC22A9 / OAT7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 26kDa/62kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 26kDa/62kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3.35 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID114571
  • Target name
    SLC22A9
  • Target description
    solute carrier family 22 member 9
  • Target synonyms
    HOAT4, OAT4, OAT7, UST3H, ust3, organic anion transporter 7, organic anion transporter 4, organic anion/short-chain fatty acid exchanger, solute carrier family 22 (organic anion transporter), member 9, solute carrier family 22 (organic anion/cation transporter), member 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC22A9 using anti-SLC22A9 antibody (A08762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A9 antigen affinity purified polyclonal antibody (Catalog # A08762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A9 at approximately 70 kDa. The expected band size for SLC22A9 is at 62 kDa.
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