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SLC27A3 Antibody

CSB-PA021535GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC27A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC27A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11000
  • Target name
    SLC27A3
  • Target description
    solute carrier family 27 member 3
  • Target synonyms
    ACSVL3; arachidonate--CoA ligase; FATP3; long-chain fatty acid transport protein 3; solute carrier family 27 (fatty acid transporter), member 3; solute carrier family 27 member 3; very long-chain acyl-CoA synthetase homolog 3; VLCS-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5K4L6
  • Protein Name
    Long-chain fatty acid transport protein 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP3/SLC27A3 antigen affinity purified polyclonal antibody (Catalog # A12345-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP3/SLC27A3 at approximately 70-79 kDa. The expected band size for FATP3/SLC27A3 is at 74 kDa.
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