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ProductsBack/SLC27A3 antibody, Internal
WB analysis of MCF-7 cells using GTX46793 SLC27A3 antibody at 1microg/ml.
WB analysis of MCF-7 cells using GTX46793 SLC27A3 antibody at 1microg/ml.
WB analysis of MCF-7 cells using GTX46793 SLC27A3 antibody at 1microg/ml.

SLC27A3 antibody, Internal

GTX46793
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSLC27A3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC27A3 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11000
  • Target name
    SLC27A3
  • Target description
    solute carrier family 27 member 3
  • Target synonyms
    ACSVL3; arachidonate--CoA ligase; FATP3; long-chain fatty acid transport protein 3; solute carrier family 27 (fatty acid transporter), member 3; solute carrier family 27 member 3; very long-chain acyl-CoA synthetase homolog 3; VLCS-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5K4L6
  • Protein Name
    Long-chain fatty acid transport protein 3
  • Scientific Description
    This gene belongs to a family of integral membrane proteins and encodes a protein that is involved in lipid metabolism. The increased expression of this gene in human neural stem cells derived from induced pluripotent stem cells suggests that it plays an important role in early brain development. Naturally occurring mutations in this gene are associated with autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP3/SLC27A3 antigen affinity purified polyclonal antibody (Catalog # A12345-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP3/SLC27A3 at approximately 70-79 kDa. The expected band size for FATP3/SLC27A3 is at 74 kDa.
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