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ProductsBack/SLC2A10 / GLUT10 Antibody (aa371-420)

SLC2A10 / GLUT10 Antibody (aa371-420)

LS-C40443
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSLC2A10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC2A10 / GLUT10 Antibody (aa371-420)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (2.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81031
  • Target name
    SLC2A10
  • Target description
    solute carrier family 2 member 10
  • Target synonyms
    ATORS, ATS, GLUT10, solute carrier family 2, facilitated glucose transporter member 10, GLUT-10, facilitative glucose transporter GLUT10, glucose transporter type 10, solute carrier family 2 (facilitated glucose transporter), member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GLUT10/SLC2A10 using anti-GLUT10/SLC2A10 antibody (A06461-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT10/SLC2A10 antigen affinity purified polyclonal antibody (Catalog # A06461-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT10/SLC2A10 at approximately 67KD. The expected band size for GLUT10/SLC2A10 is at 54KD.
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