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Western Blot analysis of SH-SY5Y cells using Glut3 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using Glut3 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using Glut3 Polyclonal Antibody

SLC2A3 Antibody

CSB-PA050080
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC2A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC2A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6515
  • Target name
    SLC2A3
  • Target description
    solute carrier family 2 member 3
  • Target synonyms
    GLUT3, solute carrier family 2, facilitated glucose transporter member 3, GLUT-3, glucose transporter type 3, brain, solute carrier family 2 (facilitated glucose transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11169
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GLUT3 using anti-GLUT3 antibody (M03259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT3 antigen affinity purified monoclonal antibody (Catalog # M03259) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT3 at approximately 54 kDa. The expected band size for GLUT3 is at 54 kDa.
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