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Western blot analysis on LOVO cell lysate using GLUT3 Antibody
Western blot analysis on LOVO cell lysate using GLUT3 Antibody
Western blot analysis on LOVO cell lysate using GLUT3 Antibody

SLC2A3 Antibody

CSB-PA868995
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC2A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC2A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6515
  • Target name
    SLC2A3
  • Target description
    solute carrier family 2 member 3
  • Target synonyms
    GLUT3, solute carrier family 2, facilitated glucose transporter member 3, GLUT-3, glucose transporter type 3, brain, solute carrier family 2 (facilitated glucose transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11169
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 3
  • Scientific Description
    GLUT3 an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is induced by insulin. It is restricted in humans to brain, testis (in the spermatozoa), and skeletal muscle, mostly in triads of slow-twitch fibres. Detected only in the brain of mouse and rat.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GLUT3 using anti-GLUT3 antibody (M03259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT3 antigen affinity purified monoclonal antibody (Catalog # M03259) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT3 at approximately 54 kDa. The expected band size for GLUT3 is at 54 kDa.
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