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Western Blot analysis of rat kidney K562 cells using NPT2b Polyclonal Antibody
Western Blot analysis of rat kidney K562 cells using NPT2b Polyclonal Antibody
Western Blot analysis of rat kidney K562 cells using NPT2b Polyclonal Antibody

SLC34A2 Antibody

CSB-PA004755
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetSLC34A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC34A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10568
  • Target name
    SLC34A2
  • Target description
    solute carrier family 34 member 2
  • Target synonyms
    NAPI-3B, NAPI-IIb, NPTIIb, NaPi2b, PULAM, sodium-dependent phosphate transport protein 2B, sodium/phosphate cotransporter 2B, solute carrier family 34 (sodium phosphate), member 2, solute carrier family 34 (type II sodium/phosphate cotransporter), member 2, type II sodium-dependent phosphate transporter 3b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95436
  • Protein Name
    Sodium-dependent phosphate transport protein 2B
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC34A2 antigen affinity purified polyclonal antibody (Catalog # A03957-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC34A2 at approximately 76-130KD. The expected band size for SLC34A2 is at 76KD.
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