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ProductsBack/SLC34A2 / NaPi-2b Antibody

SLC34A2 / NaPi-2b Antibody

LS-C410995
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSLC34A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC34A2 / NaPi-2b Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 75kDa, while the observed MW by Western blot was 76kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 75kDa, while the observed MW by Western blot was 76kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10568
  • Target name
    SLC34A2
  • Target description
    solute carrier family 34 member 2
  • Target synonyms
    NAPI-3B, NAPI-IIb, NPTIIb, NaPi2b, PULAM, sodium-dependent phosphate transport protein 2B, sodium/phosphate cotransporter 2B, solute carrier family 34 (sodium phosphate), member 2, solute carrier family 34 (type II sodium/phosphate cotransporter), member 2, type II sodium-dependent phosphate transporter 3b
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC34A2 antigen affinity purified polyclonal antibody (Catalog # A03957-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC34A2 at approximately 76-130KD. The expected band size for SLC34A2 is at 76KD.
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