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ProductsBack/SLC35D1 Antibody (aa179-227)

SLC35D1 Antibody (aa179-227)

LS-C186822
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSLC35D1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC35D1 Antibody (aa179-227)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23169
  • Target name
    SLC35D1
  • Target description
    solute carrier family 35 member D1
  • Target synonyms
    SHNKND, UGTREL7, nucleotide sugar transporter SLC35D1, UDP-GlcA/UDP-GalNAc transporter, UDP-galactose transporter-related 7, UDP-galactose transporter-related protein 7, UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1, solute carrier family 35 (UDP-glucuronic acid/UDP-N-acetylgalactosamine dual transporter), member D1
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC35D1 using anti-SLC35D1 antibody (A11935-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35D1 antigen affinity purified polyclonal antibody (Catalog # A11935-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35D1 at approximately 39 kDa. The expected band size for SLC35D1 is at 39 kDa.
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