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ProductsBack/SLC3A1 / ATR1 Antibody

SLC3A1 / ATR1 Antibody

LS-C334095
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSLC3A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC3A1 / ATR1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 35kDa/45kDa/46kDa/57kDa/63kDa/65kDa/78kDa, while the observed MW by Western blot was 78kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 35kDa/45kDa/46kDa/57kDa/63kDa/65kDa/78kDa, while the observed MW by Western blot was 78kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6519
  • Target name
    SLC3A1
  • Target description
    solute carrier family 3 member 1
  • Target synonyms
    ATR1, CSNU1, D2H, NBAT, RBAT, amino acid transporter heavy chain SLC3A1, B(0,+)-type amino acid transport protein, amino acid transporter 1, b(0,+)-type amino acid transporter-related heavy chain, neutral and basic amino acid transport protein rBAT, solute carrier family 3 (amino acid transporter heavy chain), member 1, solute carrier family 3 (cystine, dibasic and neutral amino acid transporters), member 1, solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC3A1 using anti-SLC3A1 antibody (A02654-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse HBZY whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC3A1 antigen affinity purified polyclonal antibody (Catalog # A02654-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC3A1 at approximately 110 kDa. The expected band size for SLC3A1 is at 79 kDa.
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