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Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: SLC9A3R2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 37, 25 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: SLC9A3R2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 37, 25 kDa Observed band size: 38 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: SLC9A3R2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 37, 25 kDa Observed band size: 38 kDa

SLC9A3R2 Antibody

CSB-PA01759A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC9A3R2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC9A3R2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9351
  • Target name
    SLC9A3R2
  • Target description
    SLC9A3 regulator 2
  • Target synonyms
    E3KARP; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; NHE3 kinase A regulatory protein E3KARP; NHE3 regulatory factor 2; NHE3RF2; NHERF2; NHERF-2; OCTS2; SIP1; SIP-1; sodium/hydrogen exchanger 3 kinase A regulatory protein; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2; SRY-interacting protein 1; TKA-1; tyrosine kinase activator protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15599
  • Protein Name
    Na(+)/H(+) exchange regulatory cofactor NHE-RF2
  • Scientific Description
    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3 (PubMed:18829453). May also act as scaffold protein in the nucleus.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A3R2 antigen affinity purified polyclonal antibody (Catalog # A05624-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A3R2 at approximately 45 kDa. The expected band size for SLC9A3R2 is at 37 kDa.
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