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Slingshot-1L (C-terminal region) Antibody

BS-70664R
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSSH1
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Overview

  • Supplier
    Bioss
  • Product Name
    Slingshot-1L (C-terminal region) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54434
  • Target name
    SSH1
  • Target description
    slingshot protein phosphatase 1
  • Target synonyms
    SSH1L, protein phosphatase Slingshot homolog 1, SSH-like protein 1, hSSH-1L, slingshot homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYL5
  • Protein Name
    Protein phosphatase Slingshot homolog 1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSH1 using anti-SSH1 antibody (A03480). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH1 antigen affinity purified polyclonal antibody (Catalog # A03480) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH1 at approximately 150 kDa. The expected band size for SSH1 is at 116 kDa.
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