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Slit2 Rabbit pAb

A15353
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSLIT2
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Overview

  • Supplier
    ABclonal
  • Product Name
    Slit2 Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9353
  • Target name
    SLIT2
  • Target description
    slit guidance ligand 2
  • Target synonyms
    SLIL3; slit homolog 2 protein; Slit-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94813
  • Protein Name
    Slit homolog 2 protein
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLIT2 using anti-SLIT2 antibody (A01627-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLIT2 antigen affinity purified polyclonal antibody (Catalog # A01627-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLIT2 at approximately 170 kDa. The expected band size for SLIT2 is at 170 kDa.
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