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WB analysis of human brain tissue lysate using GTX31333 SLITRK1 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human brain tissue lysate using GTX31333 SLITRK1 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human brain tissue lysate using GTX31333 SLITRK1 antibody. Working concentration : (A) 1 and (B) 2 microg/ml

SLITRK1 antibody

GTX31333
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLITRK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLITRK1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. IHC-P: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID114798
  • Target name
    SLITRK1
  • Target description
    SLIT and NTRK like family member 1
  • Target synonyms
    LRRC12, TTM, SLIT and NTRK-like protein 1, leucine-rich repeat-containing protein 12, slit and trk like gene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PX8
  • Protein Name
    SLIT and NTRK-like protein 1
  • Scientific Description
    This intronless gene encodes a member of the SLITRK protein family. These proteins are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. However, the protein encoded by this gene lacks the region of homology to neurotrophin receptors. The protein is thought to be involved in neurite outgrowth. Mutations in this gene may be associated with Tourette syndrome.[provided by RefSeq, Mar 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLITRK1 using anti-SLITRK1 antibody (A07507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLITRK1 antigen affinity purified polyclonal antibody (Catalog # A07507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLITRK1 at approximately 55 kDa. The expected band size for SLITRK1 is at 78 kDa.
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