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Western Blot analysis of COLO205 cells using SLK Polyclonal Antibody
Western Blot analysis of COLO205 cells using SLK Polyclonal Antibody
Western Blot analysis of COLO205 cells using SLK Polyclonal Antibody

SLK Antibody

CSB-PA004103
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLK
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9748
  • Target name
    SLK
  • Target description
    STE20 like kinase
  • Target synonyms
    LOSK, STK2, bA16H23.1, se20-9, STE20-like serine/threonine-protein kinase, CTCL tumor antigen se20-9, Long Ste20-like Kinase, SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase, SNF1 sucrose nonfermenting like kinase, STE20-related kinase, STE20-related serine/threonine-protein kinase, Ste20-related serine/threonine kinase, hSLK, serine/threonine-protein kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2G2
  • Protein Name
    STE20-like serine/threonine-protein kinase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SLK using anti-SLK antibody (A01614-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLK antigen affinity purified polyclonal antibody (Catalog # A01614-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLK at approximately 245 kDa. The expected band size for SLK is at 143 kDa.
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