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SLK Antibody

LS-C409133
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSLK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLK Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 138kDa/142kDa, while the observed MW by Western blot was 250kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 138kDa/142kDa, while the observed MW by Western blot was 250kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9748
  • Target name
    SLK
  • Target description
    STE20 like kinase
  • Target synonyms
    LOSK, STK2, bA16H23.1, se20-9, STE20-like serine/threonine-protein kinase, CTCL tumor antigen se20-9, Long Ste20-like Kinase, SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase, SNF1 sucrose nonfermenting like kinase, STE20-related kinase, STE20-related serine/threonine-protein kinase, Ste20-related serine/threonine kinase, hSLK, serine/threonine-protein kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLK using anti-SLK antibody (A01614-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLK antigen affinity purified polyclonal antibody (Catalog # A01614-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLK at approximately 245 kDa. The expected band size for SLK is at 143 kDa.
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