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WB analysis of various sample lysates using GTX32879 SLUG antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32879 SLUG antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32879 SLUG antibody. Dilution : 1:1000 Loading : 25microg per lane

SLUG antibody

GTX32879
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSNAI2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLUG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6591
  • Target name
    SNAI2
  • Target description
    snail family transcriptional repressor 2
  • Target synonyms
    neural crest transcription factor SLUG; protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; SLUGH; SLUGH1; snail family zinc finger 2; snail homolog 2; SNAIL2; WS2D; zinc finger protein SNAI2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43623
  • Protein Name
    Zinc finger protein SNAI2
  • Scientific Description
    This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.
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