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ProductsBack/SNAI2 Polyclonal Antibody
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining

SNAI2 Polyclonal Antibody

BS-1382R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
TargetSNAI2
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Overview

  • Supplier
    Bioss
  • Product Name
    SNAI2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6591
  • Target name
    SNAI2
  • Target description
    snail family transcriptional repressor 2
  • Target synonyms
    SLUG, SLUGH, SLUGH1, SNAIL2, WS2D, zinc finger protein SNAI2, neural crest transcription factor SLUG, protein snail homolog 2, slug (chicken homolog), zinc finger protein, snail family zinc finger 2, snail homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43623
  • Protein Name
    Zinc finger protein SNAI2
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li Z, Duan H, Li W, et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019,184:227-233. doi: 10.1016/j.exer.2019.04.012
    Read this paper
  • Bai JW, Chen MN, Wei XL, et al. The zinc-finger transcriptional factor Slug transcriptionally downregulates ERα by recruiting lysine-specific demethylase 1 in human breast cancer. Oncogenesis. 2017,6(5):e330. doi: 10.1038/oncsis.2017.38
    Read this paper

Datasheet

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Figure 1. Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.
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