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ProductsBack/SNAI2 Polyclonal Antibody
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon cancer labeled with Anti-SNAI2/SLUG Polyclonal Antibody, Unconjugated (bs-1382R) followed by conjugation to the secondary antibody and DAB staining

SNAI2 Polyclonal Antibody

BS-1382R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSNAI2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SLUG Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6591
  • Target name
    SNAI2
  • Target description
    snail family transcriptional repressor 2
  • Target synonyms
    neural crest transcription factor SLUG; protein snail homolog 2; SLUG; slug (chicken homolog), zinc finger protein; SLUGH; SLUGH1; snail family zinc finger 2; snail homolog 2; SNAIL2; WS2D; zinc finger protein SNAI2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43623
  • Protein Name
    Zinc finger protein SNAI2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Li Z et al., 2019 Jul, Exp Eye Res
    Read more
  • The zinc-finger transcriptional factor Slug transcriptionally downregulates ERalpha by recruiting lysine-specific demethylase 1 in human breast cancer. Bai JW et al., 2017 May 8, Oncogenesis
    Read more

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Figure 1. Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.
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