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ProductsBack/SLUG (SNAI2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (RC202365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2. Positive lysates LY401070 (100ug) and LC401070 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (RC202365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2. Positive lysates LY401070 (100ug) and LC401070 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (RC202365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2. Positive lysates LY401070 (100ug) and LC401070 (20ug) can be purchased separately from OriGene.

SLUG (SNAI2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G7]

TA800196BM
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSNAI2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SLUG (SNAI2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1G7
  • Gene ID6591
  • Target name
    SNAI2
  • Target description
    snail family transcriptional repressor 2
  • Target synonyms
    SLUG, SLUGH, SLUGH1, SNAIL2, WS2D, zinc finger protein SNAI2, neural crest transcription factor SLUG, protein snail homolog 2, slug (chicken homolog), zinc finger protein, snail family zinc finger 2, snail homolog 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43623
  • Protein Name
    Zinc finger protein SNAI2
  • Scientific Description
    SNAI2 mouse monoclonal antibody,clone 1G7, HRP conjugated
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.
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