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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.

Smad2 Polyclonal Antibody

bs-0718R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Rabbit, Rat
TargetSMAD2
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Overview

  • Supplier
    Bioss
  • Product Name
    Smad2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4087
  • Target name
    SMAD2
  • Target description
    SMAD family member 2
  • Target synonyms
    CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, mothers against decapentaplegic homolog 2, MAD homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15796
  • Protein Name
    Mothers against decapentaplegic homolog 2
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zheng HX, Qi SS, He J, et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020,68(15):4399-4410. doi: 10.1021/acs.jafc.0c00680
    Read this paper
  • Zhou C, Liu J, Ge Y, et al. Remote Ischemic Preconditioning Ameliorates Renal Fibrosis After Ischemia-Reperfusion Injury via Transforming Growth Factor beta1 (TGF-β1) Signalling Pathway in Rats. Med Sci Monit. 2020,26:e919185. doi: 10.12659/MSM.919185
    Read this paper
  • Han B, Lv Z, Zhang X, et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2020,259:113870. doi: 10.1016/j.envpol.2019.113870
    Read this paper
  • Yan Y, Liu X, Gao J, et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020,25(1). doi: 10.3390/molecules25010192
    Read this paper
  • Lv Y, Bing Q, Lv Z, et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2020,705:135915. doi: 10.1016/j.scitotenv.2019.135915
    Read this paper
  • Ge Y, Li S, Hu XY, et al. TCEA3 promotes differentiation of C2C12 cells via an Annexin A1-mediated transforming growth factor-β signaling pathway. J Cell Physiol. 2019,234(7):10554-10565. doi: 10.1002/jcp.27726
    Read this paper
  • Zhao XX, An XL, Zhu XC, et al. Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells. J Exp Zool B Mol Dev Evol. 2018,330(8):406-416. doi: 10.1002/jez.b.22836
    Read this paper
  • Chen X, Qin Z, Zhao J, et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats. J Biomed Nanotechnol. 2018,14(12):2135-2145. doi: 10.1166/jbn.2018.2644
    Read this paper
  • Zhang H, Ju B, Nie Y, et al. Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells. Int J Mol Med. 2018,42(1):279-289. doi: 10.3892/ijmm.2018.3600
    Read this paper
  • Zhou Y, Zhang Q, Gao Y, et al. Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway. Int J Mol Med. 2018,41(1):473-484. doi: 10.3892/ijmm.2017.3199
    Read this paper

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