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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4°C, DAB staining.

Smad2 Polyclonal Antibody

bs-0718R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Goat, Human, Mouse, Rabbit, Rat, Other Species
TargetSMAD2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Smad2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4087
  • Target name
    SMAD2
  • Target description
    SMAD family member 2
  • Target synonyms
    hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; MADH2; MADR2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15796
  • Protein Name
    Mothers against decapentaplegic homolog 2
  • Reactivity
    Bovine, Canine, Chicken, Goat, Human, Mouse, Rabbit, Rat, Other Species
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor beta1/Smad Expression. Zheng HX et al., 2020 Apr 15, J Agric Food Chem
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  • Remote Ischemic Preconditioning Ameliorates Renal Fibrosis After Ischemia-Reperfusion Injury via Transforming Growth Factor beta1 (TGF-beta1) Signalling Pathway in Rats. Zhou C et al., 2020 Feb 6, Med Sci Monit
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  • Deltamethrin induces liver fibrosis in quails via activation of the TGF-beta1/Smad signaling pathway. Han B et al., 2020 Apr, Environ Pollut
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  • Inhibition of TGF-beta Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Yan Y et al., 2020 Jan 2, Molecules
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  • Imidacloprid-induced liver fibrosis in quails via activation of the TGF-beta1/Smad pathway. Lv Y et al., 2020 Feb 25, Sci Total Environ
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  • TCEA3 promotes differentiation of C2C12 cells via an Annexin A1-mediated transforming growth factor-beta signaling pathway. Ge Y et al., 2019 Jul, J Cell Physiol
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  • Inhibiting transforming growth factor-beta signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells. Zhao XX et al., 2018 Dec, J Exp Zool B Mol Dev Evol
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  • Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats. Chen X et al., 2018 Dec 1, J Biomed Nanotechnol
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  • Adenovirus-mediated knockdown of activin A receptor type 2A attenuates immune-induced hepatic fibrosis in mice and inhibits interleukin-17-induced activation of primary hepatic stellate cells. Zhang H et al., 2018 Jul, Int J Mol Med
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  • Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-beta1/Smad pathway. Zhou Y et al., 2018 Jan, Int J Mol Med
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