More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SMAD5 Mouse Monoclonal Antibody [Clone ID: 3H9]
Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.

SMAD5 Mouse Monoclonal Antibody [Clone ID: 3H9]

AM06562SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSMAD5
Price on request
100 ul
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    SMAD5 Mouse Monoclonal Antibody [Clone ID: 3H9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H9
  • Gene ID4090
  • Target name
    SMAD5
  • Target description
    SMAD family member 5
  • Target synonyms
    DWFC, JV5-1, MADH5, mothers against decapentaplegic homolog 5, MAD, mothers against decapentaplegic homolog 5, SMA- and MAD-related protein 5, SMAD, mothers against DPP homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99717
  • Protein Name
    Mothers against decapentaplegic homolog 5
  • Scientific Description
    SMAD5 mouse monoclonal antibody, clone 3H9, Ascites
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biosynth
SMAD5 antibody10R-10715
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SMAD5 Antibody144-01947
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Anti-SMAD5 Antibody
Product group Antibodies
Antibodies.com
Anti-SMAD5 AntibodyA13765
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SMAD5 AntibodyLS-C749038
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD5
  • SizePrice
Figure 1. Western blot analysis of SMAD5 using anti-SMAD5 antibody (A01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4:monkey COS-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 antigen affinity purified polyclonal antibody (Catalog # A01423) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5 at approximately 52-60 kDa. The expected band size for SMAD5 is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-SMAD5 Antibody Picoband(r)A01423-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetSMAD5
  • SizePrice
Product group Antibodies
Biomatik
SMAD5 Polyclonal AntibodyCAC14569
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetSMAD5
  • SizePrice
Western Blot analysis of K562 cells using Smad5 Polyclonal Antibody
Product group Antibodies
Cusabio
SMAD5 AntibodyCSB-PA004732
ApplicationsWestern Blot, ELISA
ReactivityHuman, Rat
TargetSMAD5
  • SizePrice
Mouse testis lysates probed with SMAD5 Polyclonal Antibody, Unconjugated (bs-13890R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
SMAD5 Polyclonal AntibodyBS-13890R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetSMAD5
  • SizePrice
Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Product group Antibodies
Atlas Antibodies
Anti-SMAD5 AntibodyHPA058931
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSMAD5
  • SizePrice
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD5(GTX105101) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Product group Antibodies
GeneTex
SMAD5 antibody [N2C2], InternalGTX105101
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
ReactivityHuman
TargetSMAD5
  • SizePrice