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Western blot analysis of extracts of Rat lung using Smad5 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat lung using Smad5 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat lung using Smad5 Polyclonal Antibody at 1:1000 dilution.

SMAD5 Polyclonal Antibody

E-AB-60517
Elabscience
Product group Antibodies

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Overview

  • Supplier
    Elabscience
  • Product Name
    SMAD5 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMAD5 using anti-SMAD5 antibody (A01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4:monkey COS-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 antigen affinity purified polyclonal antibody (Catalog # A01423) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5 at approximately 52-60 kDa. The expected band size for SMAD5 is at 52 kDa.
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