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SMARCD1 Antibody

CAB6310
Assay Genie
TargetSMARCD1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SMARCD1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6602
  • Target name
    SMARCD1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit D1
  • Target synonyms
    BAF60A, CRACD1, CSS11, Rsc6p, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, SWI/SNF complex 60 kDa subunit A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1, Swp73-like protein, chromatin remodeling complex BAF60A subunit, mammalian chromatin remodeling complex BRG1-associated factor 60A
  • Host
    Rabbit
  • Protein IDQ96GM5
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: SMARCD1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 54 kDa Observed band size: 59 kDa
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Figure 1. Western blot analysis of SMARCD1 using anti-SMARCD1 antibody (A07318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD1 antigen affinity purified polyclonal antibody (Catalog # A07318-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCD1 at approximately 58 kDa. The expected band size for SMARCD1 is at 58 kDa.
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