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ProductsBack/SMARCE1 / BAF57 Antibody (N-Terminus)

SMARCE1 / BAF57 Antibody (N-Terminus)

LS-B11494
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSMARCE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMARCE1 / BAF57 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml), WB (0.2 - 1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6605
  • Target name
    SMARCE1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit E1
  • Target synonyms
    BAF57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, chromatin remodeling complex BRG1-associated factor 57
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAF57/SMARCE1 antigen affinity purified polyclonal antibody (Catalog # A03739-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAF57/SMARCE1 at approximately 54 kDa. The expected band size for BAF57/SMARCE1 is at 47 kDa.
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