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ProductsBack/SMO / Smoothened Antibody

SMO / Smoothened Antibody

LS-C408690
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSMO
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMO / Smoothened Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 86kDa, while the observed MW by Western blot was 86kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 86kDa, while the observed MW by Western blot was 86kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6608
  • Target name
    SMO
  • Target description
    smoothened, frizzled class receptor
  • Target synonyms
    CRJS; frizzled family member 11; FZD11; Gx; PHLS; protein Gx; seven transmembrane helix receptor; SMOH; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Smoothened/SMO using anti-Smoothened/SMO antibody (A00451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothened/SMO antigen affinity purified polyclonal antibody (Catalog # A00451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothened/SMO at approximately 86 kDa. The expected band size for Smoothened/SMO is at 86 kDa.
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