Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled mouse brain extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640362/GTX640362_T-45411_20240524_WB_M_brain_24052802_973.webp "Boiled and unboiled mouse brain extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640362/GTX640362_T-45411_20240712_WB_KO_watermark_24071520_850.webp "Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX640362/GTX640362_45502_20240816_WB_Fraction_ub_24082301_622.webp "Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![Smoothened homolog antibody [HL2975] (GTX640362) specifically detects Smoothened homolog protein on a VirD?-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:2000 dilution. Yellow: SMO](https://www.genetex.com/upload/website/prouct_img/normal/GTX640362/GTX640362_45502_20250514_GPCRarray_25051403_772.webp "Smoothened homolog antibody [HL2975] (GTX640362) specifically detects Smoothened homolog protein on a VirD?-GPCR array (CDI Labs, Baltimore, MD). Array was probed with antibody using a 1:2000 dilution. Yellow: SMO")
![Smoothened homolog antibody [HL2975] (GTX640362) detects Smoothened homolog protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640362/GTX640362_45502_20250919_FCM_25100122_398.webp "Smoothened homolog antibody [HL2975] (GTX640362) detects Smoothened homolog protein by flow cytometry analysis. Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Smoothened homolog antibody [HL2975]
GTX640362
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSMO
Overview
- SupplierGeneTex
- Product NameSmoothened homolog antibody [HL2975]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2975
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6608
- Target nameSMO
- Target descriptionsmoothened, frizzled class receptor
- Target synonymsCRJS, FZD11, Gx, PHLS, SMOH, protein smoothened, frizzled family member 11, protein Gx, seven transmembrane helix receptor, smoothened homolog, smoothened, frizzled family receptor, smoothened, seven transmembrane spanning receptor
- HostRabbit
- IsotypeIgG
- Protein IDQ99835
- Protein NameProtein smoothened
- Scientific DescriptionThe protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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