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ProductsBack/SMRT antibody [1542]

SMRT antibody [1542]

GTX70248
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
DownloadDatasheet
Product group Antibodies
TargetNCOR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMRT antibody [1542]
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1542
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9612
  • Target name
    NCOR2
  • Target description
    nuclear receptor corepressor 2
  • Target synonyms
    CTG repeat protein 26; CTG26; N-CoR2; nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; SMAP270; SMRT; SMRTE; SMRTE-tau; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TNRC14; TRAC; TRAC1; TRAC-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y618
  • Protein Name
    Nuclear receptor corepressor 2
  • Scientific Description
    This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Repressors of androgen and progesterone receptor action. Agoulnik IU et al., 2003 Aug 15, J Biol Chem
    Read more
  • Regulation of androgen receptor activity by the nuclear receptor corepressor SMRT. Liao G et al., 2003 Feb 14, J Biol Chem
    Read more
  • SMRTE inhibits MEF2C transcriptional activation by targeting HDAC4 and 5 to nuclear domains. Wu X et al., 2001 Jun 29, J Biol Chem
    Read more

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Figure 1. Western blot analysis of NCOR2 using anti-NCOR2 antibody (A01412-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR2 antigen affinity purified polyclonal antibody (Catalog # A01412-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOR2 at approximately 275 kDa. The expected band size for NCOR2 is at 275 kDa.
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