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Smt3 Polyclonal Antibody

CAC12806
Biomatik
ApplicationsELISA
Product group Antibodies
ReactivityYeast
TargetSMT3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Smt3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID852122
  • Target name
    SMT3
  • Target description
    SUMO family protein SMT3
  • Target synonyms
    YDR510W, SUMO family protein SMT3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12306
  • Protein Name
    Ubiquitin-like protein SMT3
  • Scientific Description
    The Smt3 Polyclonal Antibody (Species: Saccharomyces cerevisiae (Bakers yeast)) has been validated for the following applications: ELISA.
  • Reactivity
    Yeast
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 μg E.coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 μg total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60o C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG.
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