Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638782/GTX638782_T-45089_20230721_IHC-P_multiple_R_23073119_865.webp "SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638782/GTX638782_T-45089_20230721_IHC-P_M_23073119_245.webp "SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638782/GTX638782_45159_20230908_WB_Z_23091319_909.webp "Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638782/GTX638782_45159_20230929_WB_TPM_watermark_23100319_474.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![SMYD3 antibody [HL2464] detects SMYD3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638782/GTX638782_45159_20240607_ICC_IF_24062501_970.webp "SMYD3 antibody [HL2464] detects SMYD3 protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
SMYD3 antibody [HL2464]
GTX638782
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetSMYD3
Overview
- SupplierGeneTex
- Product NameSMYD3 antibody [HL2464]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2464
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID64754
- Target nameSMYD3
- Target descriptionSET and MYND domain containing 3
- Target synonymsKMT3E, ZMYND1, ZNFN3A1, bA74P14.1, histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, zinc finger MYND domain-containing protein 1, zinc finger protein, subfamily 3A (MYND domain containing), 1, zinc finger, MYND domain containing 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9H7B4
- Protein NameHistone-lysine N-methyltransferase SMYD3
- Scientific DescriptionThis gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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